2AAM
Crystal structure of a putative glycosidase (tm1410) from thermotoga maritima at 2.20 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979170, 0.918370 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 194.930, 84.620, 195.010 |
Unit cell angles | 90.00, 119.90, 90.00 |
Refinement procedure
Resolution | 28.590 - 2.200 |
Rwork | 0.216 |
R-free | 0.23900 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.529 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.590 | 28.590 | 2.280 |
High resolution limit [Å] | 2.200 | 4.720 | 2.200 |
Rmerge | 0.093 | 0.543 | 0.543 |
Number of reflections | 263407 | 53751 | 34138 |
<I/σ(I)> | 8.15 | 17.44 | 1.97 |
Completeness [%] | 90.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 9.5 | 277 | 25.0% PEG-300, 10.0% Glycerol, 5.0% PEG-8000, 0.1M CHES pH 9.5 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |