Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-01 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.800, 44.900, 83.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.400 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.28700 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.800 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.077 * |
| Total number of observations | 40453 * |
| Number of reflections | 6377 |
| Completeness [%] | 97.9 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.7 | Cook, W.J., (1992) J.Mol.Biol., 223, 1183. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 17.5 (%(w/v)sat) | |
| 3 | 1 | drop | MES | 0.025 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 35 (%sat) | |
| 5 | 1 | reservoir | MES | 0.05 (M) |
