2AAE

THE ROLE OF HISTIDINE-40 IN RIBONUCLEASE T1 CATALYSIS: THREE-DIMENSIONAL STRUCTURES OF THE PARTIALLY ACTIVE HIS40LYS MUTANT

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	48.270, 46.500, 41.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.145
RMSD bond length	0.018
RMSD bond angle	0.060 *
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.063 *
Completeness [%]		77 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/mL)
2	1	drop	Tris acetate	20 (mM)
3	1	drop	sodium acetate	5 (mM)
4	1	drop	calcium acetate	2 (mM)
5	1	reservoir	PEG4000	40 (%(w/v))