2AAC
ESCHERCHIA COLI GENE REGULATORY PROTEIN ARAC COMPLEXED WITH D-FUCOSE
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-09 |
Detector | FUJI |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.247, 93.682, 49.915 |
Unit cell angles | 90.00, 95.57, 90.00 |
Refinement procedure
Resolution | 7.000 - 1.600 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.22200 |
Structure solution method | RIGID-BODY REFINEMENT AND DIFFERENCE FOURIER MAPS |
Starting model (for MR) | 2arc |
RMSD bond length | 0.010 |
RMSD bond angle | 24.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.670 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.090 * | 0.080 |
Total number of observations | 293876 * | |
Number of reflections | 57378 * | |
<I/σ(I)> | 9.7 | 2.5 |
Completeness [%] | 99.9 | 99 |
Redundancy | 5.2 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.25 * | used to seeding, Soisson, S.M., (1997) Science, 276, 421. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 18 (%) | |
2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
3 | 1 | reservoir | magnesium acetate | 40 (mM) |