2AAB

Structural basis of antigen mimicry in a clinically relevant melanoma antigen system

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-01-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	75.050, 76.890, 82.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.000 - 2.500
R-factor	0.236
Rwork	0.236
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rsc
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.067
Number of reflections	16656
<I/σ(I)>		4.2
Completeness [%]	98.0	95
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	PEG 6000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K