2AA3
Crystal structure of Plasmodium vivax lactate dehydrogenase complex with APADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.482, 128.454, 130.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.670 - 2.050 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a92 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.783 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.670 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.099 | 0.379 |
| Number of reflections | 85499 | |
| <I/σ(I)> | 21.3 | 4.6 |
| Completeness [%] | 98.4 | 96.9 |
| Redundancy | 8.2 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG5k MME, Ammonium sulphate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
