2AA1
Crystal structure of the cathodic hemoglobin isolated from the Antarctic fish Trematomus Newnesi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.097, 60.801, 61.232 |
| Unit cell angles | 80.73, 81.36, 62.01 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.193 |
| Rwork | 0.186 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hbh |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Outer shell | |
| Low resolution limit [Å] | 1.880 |
| High resolution limit [Å] | 1.800 |
| Completeness [%] | 77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 9-12%(w/v) PEG 6000, phosphate buffer, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
