2A9V

Crystal structure of a putative gmp synthase subunit a protein (ta0944m) from thermoplasma acidophilum at 2.45 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00, 0.97971, 0.97947
Spacegroup name	P 21 21 2
Unit cell lengths	275.661, 39.045, 68.267
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.270 - 2.240
Rwork	0.176
R-free	0.24600
Structure solution method	MAD, Molecular replacement
Starting model (for MR)	1qdl
RMSD bond length	0.016
RMSD bond angle	1.570
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.270	66.260	2.300
High resolution limit [Å]	2.240	10.020	2.240
Rmerge	0.053	0.042	0.163
Number of reflections	29096
<I/σ(I)>	9	9.1	4.8
Completeness [%]	78.6
Redundancy	3.2	3.2	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		293	22.00% PEG 3350, 0.20M NP_Sodium Thiocyanate, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K