2A9R
RR02-Rec Phosphate in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-20 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.673, 92.829, 36.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.400 - 2.342 |
| R-factor | 0.20222 |
| Rwork | 0.198 |
| R-free | 0.24617 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nxw |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.875 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.400 |
| High resolution limit [Å] | 2.340 |
| Number of reflections | 5980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 40% PEG300, Phosphate/citrate buffer, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
