2A9N
A Mutation Designed to Alter Crystal Packing Permits Structural Analysis of a Tight-binding Fluorescein-scFv complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.382, 100.460, 61.749 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 3.000 |
| R-factor | 0.22094 |
| Rwork | 0.217 |
| R-free | 0.26607 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 2A9M |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.260 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 3.130 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 10306 | |
| Completeness [%] | 97.5 | 96.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 298 | 29-30% (w/v) PEG 8000, 0.2 M ammonium sulfate, 10 mM sodium acetate , pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
