2A9M
Structural Analysis of a Tight-binding Fluorescein-scFv; apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.900, 73.500, 70.700 |
| Unit cell angles | 90.00, 97.11, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.21219 |
| Rwork | 0.210 |
| R-free | 0.26328 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1A7N |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.319 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.153 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 27511 | |
| Completeness [%] | 94.4 | 97.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 1.6 M ammonium sulfate, 150 mM sodium citrate , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
