2A9M
Structural Analysis of a Tight-binding Fluorescein-scFv; apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.900, 73.500, 70.700 |
Unit cell angles | 90.00, 97.11, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.100 |
R-factor | 0.21219 |
Rwork | 0.210 |
R-free | 0.26328 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1A7N |
RMSD bond length | 0.011 |
RMSD bond angle | 1.319 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.153 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 27511 | |
Completeness [%] | 94.4 | 97.48 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 1.6 M ammonium sulfate, 150 mM sodium citrate , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |