2A9K

Crystal structure of the C3bot-NAD-RalA complex reveals a novel type of action of a bacterial exoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-09-30
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	35.260, 113.850, 56.310
Unit cell angles	90.00, 106.40, 90.00

Refinement procedure

Resolution	19.030 - 1.730
R-factor	0.18834
Rwork	0.186
R-free	0.22465
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g24 1uad
RMSD bond length	0.008
RMSD bond angle	1.110
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.830
High resolution limit [Å]	1.730	1.730
Number of reflections	40078
<I/σ(I)>	13.2	3.6
Completeness [%]	90.2	62.6
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M ammonium citrate pH 7.0, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K