2A96

Crystal structure of phosphate tethered PhoN of S. typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2001-10-18
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	189.870, 45.080, 112.470
Unit cell angles	90.00, 111.58, 90.00

Refinement procedure

Resolution	20.000 - 2.500
Rwork	0.170
R-free	0.21400
Structure solution method	Molecular replacement and Non-crystallographic symmetry averaging
Starting model (for MR)	1d2t
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA (/ TRUNCATE)
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.087	0.243
Number of reflections	27475
<I/σ(I)>	8.1	2.3
Completeness [%]	90.0	89.9
Redundancy	1.4	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	PEG-6000 12%, Glycerol 2%, 25mM phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K