2A8Z
Structure Of A Cold-Adapted Family 8 Xylanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8034 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 77.779, 77.779, 149.634 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 12.000 - 3.200 |
R-factor | 0.21882 |
Rwork | 0.216 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1H13 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.080 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 | 3.270 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 8337 | |
Completeness [%] | 93.3 | 94.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | PEG8000, sodium cacodylate, ammonium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |