2A8X

Crystal Structure of Lipoamide Dehydrogenase from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-26
Detector	MARRESEARCH
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	83.582, 96.677, 122.889
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.940 - 2.400
R-factor	0.1991
Rwork	0.199
R-free	0.24600
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.940	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	72840
<I/σ(I)>	9.3	1.8
Completeness [%]	96.6	92.3
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	279	50% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 279K