2A8V
RHO TRANSCRIPTION TERMINATION FACTOR/RNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.783, 31.308, 105.751 |
| Unit cell angles | 90.00, 122.98, 90.00 |
Refinement procedure
| Resolution | 14.000 - 2.400 |
| R-factor | 0.2455 |
| Rwork | 0.245 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a8v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.140 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.074 * | 0.283 * |
| Number of reflections | 14393 | |
| <I/σ(I)> | 16 | 3.6 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | 30 * | pH 4.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 10-20 (%) | |
| 3 | 1 | reservoir | ammonium acetate | 50-100 (mM) | |
| 4 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
| 5 | 1 | reservoir | 4 (mM) | ||
| 6 | 1 | reservoir | erbium acetate | 2 (mM) |
