2A8U
Crystal Structure of Human Galactosyltransferase (GTB) Complexed with Beta-Methyl Lactoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2005-04-12 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.480, 150.690, 79.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.690 |
Rwork | 0.208 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lz7 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.750 |
High resolution limit [Å] | 1.690 | 1.690 |
Number of reflections | 35513 | |
Completeness [%] | 99.7 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |