2A8T

2.1 Angstrom Crystal Structure of the Complex Between the Nuclear U8 snoRNA Decapping Nudix Hydrolase X29, Manganese and m7G-PPP-A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2005-04-03
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	50.702, 81.710, 111.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.170 - 2.100
R-factor	0.217
Rwork	0.212
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u20
RMSD bond length	0.014
RMSD bond angle	1.137
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.170	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.037	0.380
Number of reflections	27774
<I/σ(I)>	19.8	4.4
Completeness [%]	99.9	100
Redundancy	6.92	6.88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.68	293	4-5 mg/ml X29, 0.025M HEPES pH 7.68, 3/75% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K