2A8S

2.45 Angstrom Crystal Structure of the Complex Between the Nuclear SnoRNA Decapping Nudix Hydrolase X29, Manganese and GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2005-03-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	49.998, 82.553, 112.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.740 - 2.450
Rwork	0.211
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u20
RMSD bond length	0.018
RMSD bond angle	1.139
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.740	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.057	0.343
Number of reflections	16302
<I/σ(I)>	25.4	6.9
Completeness [%]	91.9	91.3
Redundancy	11.34	10.04

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.68	293	4-5 mg/ml X29, 0.025M HEPES pH 7.68, 3/75% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K