2A8S
2.45 Angstrom Crystal Structure of the Complex Between the Nuclear SnoRNA Decapping Nudix Hydrolase X29, Manganese and GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2005-03-20 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.998, 82.553, 112.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.740 - 2.450 |
Rwork | 0.211 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u20 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.139 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.740 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.057 | 0.343 |
Number of reflections | 16302 | |
<I/σ(I)> | 25.4 | 6.9 |
Completeness [%] | 91.9 | 91.3 |
Redundancy | 11.34 | 10.04 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.68 | 293 | 4-5 mg/ml X29, 0.025M HEPES pH 7.68, 3/75% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |