2A8M

Crystal Structure of Human Taspase1 (T234S mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-05
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	60.441, 89.371, 104.276
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.590 - 2.600
R-factor	0.207
Rwork	0.207
R-free	0.29000
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB (THEN SOLVE/RESOLVE)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.101	0.392
Number of reflections	16867
<I/σ(I)>	15.0097	2.96
Completeness [%]	93.6	73.1
Redundancy	5.9	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6	294	pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K