2A8L
Crystal structure of Human Taspase1 (T234A mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-03 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.195, 85.750, 104.564 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.460 - 2.000 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.25300 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB (THEN SOLVE/RESOLVE) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.075 | 0.415 |
Number of reflections | 37258 | |
<I/σ(I)> | 21.6843 | 4.067 |
Completeness [%] | 99.7 | |
Redundancy | 5.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |