2A8K
Structural and Mutational Studies of the Catalytic Domain of Colicin E5a tRNA-Specific Ribonuclease
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Detector technology | CCD |
Collection date | 2003-03-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9794, 0.9792, 0.9686 |
Spacegroup name | P 31 |
Unit cell lengths | 65.220, 65.220, 100.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.500 |
Rwork | 0.217 |
R-free | 0.23400 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.500 |
Rmerge | 0.035 |
Number of reflections | 73778 |
<I/σ(I)> | 21.3 |
Completeness [%] | 96.2 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | PEG400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |