2A8K
Structural and Mutational Studies of the Catalytic Domain of Colicin E5a tRNA-Specific Ribonuclease
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Detector technology | CCD |
| Collection date | 2003-03-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9794, 0.9792, 0.9686 |
| Spacegroup name | P 31 |
| Unit cell lengths | 65.220, 65.220, 100.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| Rwork | 0.217 |
| R-free | 0.23400 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.035 |
| Number of reflections | 73778 |
| <I/σ(I)> | 21.3 |
| Completeness [%] | 96.2 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | PEG400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
