2A8I

Crystal Structure of human Taspase1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	124.782, 126.068, 117.923
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.430 - 2.000
R-factor	0.216
Rwork	0.216
R-free	0.26000
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000	2.000
Number of reflections	109787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	278	pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K