2A8G
Structure of Avidin in complex with the ligand deoxyguanosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.307, 79.454, 43.005 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.630 - 1.990 |
| R-factor | 0.232 |
| Rwork | 0.218 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jj8 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.944 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.630 | 2.070 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Rmerge | 0.103 | 0.202 |
| Number of reflections | 15790 | |
| <I/σ(I)> | 17.4 | 1.8 |
| Completeness [%] | 92.5 | 92.5 |
| Redundancy | 4.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 50mM Na Citrate, 20% PEG 8000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
