2A8E
Three-dimensional structure of Bacillus subtilis Q45498 putative protein at resolution 2.5A. Northeast Structural Genomics Consortium target SR204.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97922 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 102.000, 165.449, 40.952 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.790 - 2.500 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.24100 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.710 |
High resolution limit [Å] | 2.000 | 2.520 |
Rmerge | 0.058 | 0.109 |
Completeness [%] | 77.4 | 78.9 |
Redundancy | 5.45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 26% PEG 4000, 0.2M Li(2)So(4), 0.1M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |