2A83

Crystal structure of hla-b*2705 complexed with the glucagon receptor (gr) peptide (residues 412-420)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 1 21 1
Unit cell lengths	50.982, 82.083, 65.316
Unit cell angles	90.00, 108.73, 90.00

Refinement procedure

Resolution	28.990 - 1.400
R-factor	0.12734
Rwork	0.126
R-free	0.14955
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jge
RMSD bond length	0.012
RMSD bond angle	1.480
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.039	0.112
Number of reflections	97993
Completeness [%]	97.9	87.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	14% PEG4000,100mM Tris/HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K