2A7W
Crystal Structure of Phosphoribosyl-ATP Pyrophosphatase from Chromobacterium violaceum (ATCC 12472). NESG TARGET CVR7
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97908,0.97933,0.96771 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 97.938, 67.544, 113.512 |
Unit cell angles | 90.00, 92.45, 90.00 |
Refinement procedure
Resolution | 29.310 - 2.800 |
R-factor | 0.2582 |
Rwork | 0.258 |
R-free | 0.27990 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.079 | 0.382 |
Number of reflections | 36861 | |
<I/σ(I)> | 17.63 | 2.53 |
Completeness [%] | 96.5 | 97.6 |
Redundancy | 4.07 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |