2A7L
Structure of the human hypothetical ubiquitin-conjugating enzyme, LOC55284
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-05 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 38.595, 60.655, 133.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.440 - 1.820 |
| R-factor | 0.26378 |
| Rwork | 0.262 |
| R-free | 0.29982 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y6l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.352 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.475 |
| Number of reflections | 27952 | |
| <I/σ(I)> | 36.68 | 1.94 |
| Completeness [%] | 93.6 | 50.4 |
| Redundancy | 6.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 3 M NaCl, 0.1 M bisTris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
