2A78
Crystal structure of the C3bot-RalA complex reveals a novel type of action of a bacterial exoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-07-30 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.940, 112.830, 56.350 |
Unit cell angles | 90.00, 105.10, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 35816 | |
<I/σ(I)> | 11.8 | 2.3 |
Completeness [%] | 91.8 | 62.9 |
Redundancy | 2.3 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M ammonium sulfate, 0.1 M bis-Tris, 25% w/v polyethylene glycol 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |