2A6Z
Crystal structure of Emp47p carbohydrate recognition domain (CRD), monoclinic crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9779 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.520, 64.990, 41.540 |
| Unit cell angles | 90.00, 96.68, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.000 |
| R-factor | 0.13 |
| Rwork | 0.130 |
| R-free | 0.16300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a6y |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.032 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.046 | 0.249 |
| Number of reflections | 99298 | |
| <I/σ(I)> | 17.8 | 4.9 |
| Completeness [%] | 96.3 | 88.7 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 283 | PEG3350, Ammonium chloride, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
