2A6Y
Crystal structure of Emp47p carbohydrate recognition domain (CRD), tetragonal crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-19 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 70.310, 70.310, 100.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.420 |
R-factor | 0.134 |
Rwork | 0.134 |
R-free | 0.19200 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 0.030 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.470 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.058 | 0.374 |
Number of reflections | 48001 | |
<I/σ(I)> | 14 | 3.6 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 13.6 | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 277 | Sodium dihydrogen phosphate, Dipotassium hydrogen phosphate, Lithium sulfate, CAPS, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 277 | Sodium dihydrogen phosphate, Dipotassium hydrogen phosphate, Lithium sulfate, CAPS, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |