2A6T
Crystal structure of S.pombe mRNA decapping enzyme Dcp2p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-31 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 61 |
Unit cell lengths | 56.481, 56.481, 301.528 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.500 |
R-factor | 0.24981 |
Rwork | 0.248 |
R-free | 0.28878 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.317 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 18700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | Na/K phosphate, PEG200, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |