2A6Q
Crystal structure of YefM-YoeB complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-21 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00000 |
Spacegroup name | P 64 |
Unit cell lengths | 88.485, 88.485, 135.150 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.190 - 2.050 |
R-factor | 0.225 |
Rwork | 0.224 |
R-free | 0.25400 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.280 | 2.090 |
High resolution limit [Å] | 2.030 | 2.020 |
Number of reflections | 38636 | |
<I/σ(I)> | 27.86 | |
Completeness [%] | 99.4 | 84.2 |
Redundancy | 11.2 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Tris-HCl, NaCl, DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Tris-HCl, NaCl, DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Tris-HCl, NaCl, DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Tris-HCl, NaCl, DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |