2A6O
Crystal Structure of the ISHp608 Transposase in Complex with Stem-loop DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.333, 71.132, 76.746 |
| Unit cell angles | 90.00, 94.44, 90.00 |
Refinement procedure
| Resolution | 44.200 - 2.600 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a6m |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.060 |
| Number of reflections | 14684 |
| <I/σ(I)> | 14.7 |
| Completeness [%] | 99.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 3350, Sodium Tartrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
