2A6M

Crystal Structure of the ISHp608 Transposase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2004-06-24
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	C 1 2 1
Unit cell lengths	124.520, 50.976, 52.921
Unit cell angles	90.00, 105.45, 90.00

Refinement procedure

Resolution	22.800 - 2.400
R-factor	0.254
Rwork	0.254
R-free	0.27200
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	2.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Rmerge	0.043
Number of reflections	12555
<I/σ(I)>	28.6
Completeness [%]	98.7
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 3350, Sodium Thiocyanate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 3350, Sodium Thiocyanate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K