2A6J
Crystal structure analysis of the anti-arsonate germline antibody 36-65
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2003-04-08 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.137, 73.955, 85.922 |
Unit cell angles | 90.00, 113.92, 90.00 |
Refinement procedure
Resolution | 100.000 - 2.700 |
R-factor | 0.228 |
Rwork | 0.225 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.791 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.095 | |
Number of reflections | 23857 | |
<I/σ(I)> | 6.91 | 1.86 |
Completeness [%] | 85.3 | 86.7 |
Redundancy | 2.54 | 1.52 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 18% PEG 8000, cacodylate, 0.05% azide , pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |