2A6C
CRYSTAL STRUCTURE OF A PUTATIVE TRANSCRIPTIONAL REGULATOR (NE_1354) FROM NITROSOMONAS EUROPAEA AT 1.90 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-02 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97929, 0.91162 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.862, 40.788, 53.393 |
| Unit cell angles | 90.00, 119.83, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| Rwork | 0.182 |
| R-free | 0.24100 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.357 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.480 | 26.480 | 1.850 |
| High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
| Rmerge | 0.095 | 0.068 | 0.516 |
| Number of reflections | 14998 | ||
| <I/σ(I)> | 3.7 | 7.4 | 1.2 |
| Completeness [%] | 97.7 | ||
| Redundancy | 3.7 | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 273 | 40.0 % MPD, 0.1M Phosphate Citrate, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 273K, pH 4.2 |
