2A6B

Crystal structure of a putative transcriptional regulator of the tena family (spr0628) from streptococcus pneumoniae r6 at 1.70 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 4 21 2
Unit cell lengths	119.908, 119.908, 47.105
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.980 - 1.700
Rwork	0.155
R-free	0.17500
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.304
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.980	29.980	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.089	0.068	0.513
Number of reflections	38398
<I/σ(I)>	4.4	7.2	1.3
Completeness [%]	100.0
Redundancy	9.4	7.3	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		273	o.2M LiCl, 20.0% PEG-3350, No Buffer pH 6.7, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 273K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		273	o.2M LiCl, 20.0% PEG-3350, No Buffer pH 6.7, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 273K