2A62
Crystal structure of mouse cadherin-8 EC1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 75.818, 75.818, 233.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 4.500 |
| Rwork | 0.271 |
| R-free | 0.34600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.786 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.660 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Number of reflections | 4479 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | PEG 8000, ches, ethylene glycol, calcium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
