2A5X
Crystal Structure of a Cross-linked Actin Dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 207.377, 54.372, 36.200 |
| Unit cell angles | 90.00, 98.62, 90.00 |
Refinement procedure
| Resolution | 90.000 - 2.490 |
| R-factor | 0.19707 |
| Rwork | 0.194 |
| R-free | 0.25011 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qz5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.524 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 13943 | |
| <I/σ(I)> | 19.4 | 8.7 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 35% MPD, 100 mM sodium acetate pH 4.7, 20 mM calcium chloride, non-detergent sulfo-betaine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
