2A5B
Avidin complexed with 8-oxodeoxyguanosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.019, 79.487, 42.909 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.700 - 2.490 |
| R-factor | 0.22331 |
| Rwork | 0.219 |
| R-free | 0.31007 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ij8 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.994 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.700 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.076 | 0.420 |
| Number of reflections | 8270 | |
| <I/σ(I)> | 23.5 | 3.1 |
| Completeness [%] | 96.3 | |
| Redundancy | 6.6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 290 | PEG 8000, Sodium Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 290.0K |
