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2A52

fluorescent protein asFP595, S158V, on-state

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsENRAF-NONIUS FR591
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2004-06-09
DetectorMARRESEARCH
Wavelength(s)1.5418
Spacegroup nameC 2 2 21
Unit cell lengths75.374, 125.250, 92.580
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 1.700
R-factor0.18285
Rwork0.181
R-free0.22162
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.009
RMSD bond angle1.463
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareMOLREP
Refinement softwareREFMAC (5.1.24)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0001.750
High resolution limit [Å]1.7001.700
Number of reflections48031
Completeness [%]99.098
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.1293PEG 3350, NaCl, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K

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