2A4X
Crystal Structure Of Mitomycin C-Binding Protein Complexed with Metal-Free Bleomycin A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.248, 52.405, 107.862 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.400 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 1.420 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.041 | 0.481 |
| Number of reflections | 52971 | |
| <I/σ(I)> | 52.7 | 4.1 |
| Completeness [%] | 91.3 | 72 |
| Redundancy | 16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | PEG8000, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
