2A4X

Crystal Structure Of Mitomycin C-Binding Protein Complexed with Metal-Free Bleomycin A2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-17
Detector	MARRESEARCH
Wavelength(s)	0.9000
Spacegroup name	P 21 21 21
Unit cell lengths	47.248, 52.405, 107.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.400
R-factor	0.222
Rwork	0.222
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	1.420
High resolution limit [Å]	1.370	1.370
Rmerge	0.041	0.481
Number of reflections	52971
<I/σ(I)>	52.7	4.1
Completeness [%]	91.3	72
Redundancy	16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	PEG8000, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K