2A4V
Crystal Structure of a truncated mutant of yeast nuclear thiol peroxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 1.12714 |
| Spacegroup name | P 32 |
| Unit cell lengths | 37.535, 37.535, 83.256 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.096 | 0.362 |
| Total number of observations | 55862 * | |
| Number of reflections | 11928 | 1171 * |
| <I/σ(I)> | 12.4 | 3.09 |
| Completeness [%] | 98.1 | 97 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 283 | Choi, J., (2005) Acta Crystallogr., F61, 659. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.4 |
| 3 | 1 | reservoir | PEG3000 | 20 (%(w/v)) | |
| 4 | 1 | reservoir | 200 (mM) |
