2A4M
Structure of Trprs II bound to ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 198 |
| Collection date | 2005-05-05 |
| Wavelength(s) | 0.9100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 212.582, 58.574, 85.150 |
| Unit cell angles | 90.00, 96.64, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yi8 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 20000 | |
| Completeness [%] | 80.0 | 80 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG4K, 0.2 M DIAMMONIUM HYDROGEN PHOSPHATE, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
