2A47
Crystal structure of amFP486 H199T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.82653 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 112.955, 112.955, 81.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.300 - 1.720 |
| R-factor | 0.172 |
| Rwork | 0.169 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a46 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.300 | 1.780 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.054 | 0.383 |
| Number of reflections | 28008 | |
| Completeness [%] | 95.2 | 97.7 |
| Redundancy | 8.4 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.7 | 298 | 0.6 M Na/K tartrate, 40 M Li2SO4, 0.1 M CHES pH 9.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
