2A40
Ternary complex of the WH2 domain of WAVE with Actin-DNAse I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 118.719, 41.598, 153.048 |
Unit cell angles | 90.00, 108.79, 90.00 |
Refinement procedure
Resolution | 47.510 - 1.800 |
R-factor | 0.1689 |
Rwork | 0.166 |
R-free | 0.21503 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1atn |
RMSD bond length | 0.013 |
RMSD bond angle | 1.464 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.510 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.096 | 0.283 |
Number of reflections | 106912 | |
<I/σ(I)> | 19.9 | 4 |
Completeness [%] | 80.6 | 69 |
Redundancy | 5.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | sodium formate, PEG3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |