2A3W
Decameric structure of human serum amyloid P-component bound to Bis-1,2-{[(Z)-2-carboxy-2-methyl-1,3-dioxane]-5-yloxycarbamoyl}-ethane
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.115879 |
| Spacegroup name | P 1 |
| Unit cell lengths | 96.712, 105.325, 146.007 |
| Unit cell angles | 86.53, 78.15, 76.78 |
Refinement procedure
| Resolution | 100.000 - 2.200 |
| R-factor | 0.19005 |
| Rwork | 0.188 |
| R-free | 0.22930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lgn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.122 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.043 | |
| Number of reflections | 269382 | |
| <I/σ(I)> | 15.5 | 3.2 |
| Completeness [%] | 97.1 | 95.6 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | PEG 4000, PEG 400, sodium acetate, calcium acetate, sorbitol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
