2A3V
Structural basis for broad DNA-specificity in integron recombination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 153 |
| Detector technology | CCD |
| Collection date | 2004-11-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 149.900, 170.200, 209.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.600 - 2.800 |
| R-factor | 0.235 |
| Rwork | 0.234 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.390 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.089 | 0.384 |
| Number of reflections | 63712 | |
| <I/σ(I)> | 14.2 | 3.33 |
| Completeness [%] | 97.4 | 91.4 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | PEG 4000, Ammonium Acetate, CaCl2, MES, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | Ammonium Acetate | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 1 | MES | ||
| 5 | 1 | 1 | water | ||
| 6 | 1 | 2 | PEG 4000 | ||
| 7 | 1 | 2 | Ammonium Acetate | ||
| 8 | 1 | 2 | CaCl2 |
