2A3T
Cu-containing nitrite reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.017 |
| Spacegroup name | H 3 |
| Unit cell lengths | 72.436, 72.436, 148.089 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.500 - 1.850 |
| R-factor | 0.15022 |
| Rwork | 0.149 |
| R-free | 0.17627 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB: 1ZV2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.461 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.200 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.079 | 0.490 |
| Number of reflections | 24723 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 295 | PEG 4000, MgCl2, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
